Journal: Scientific Reports
Article Title: Characterization of Alternaria alternata alternariol monomethyl ether with a potential antiproliferative activity by topoisomerases inhibition; molecular docking and dynamic simulations
doi: 10.1038/s41598-026-51757-8
Figure Lengend Snippet: Antiproliferative, and anti-Topoisomerase I and II activities of the purified AME of A. alternata. A , The anti-proliferative activity of the purified AME towards the different cell lines (MCF7, HepG-2 cells,Caco2, and normal OEC cells), compared to Taxol as a positive control. Kinetics of Topoisomerase I ( B ) and Topoisomerase II ( C ), inhibition at different concentrations of AME by A. alternata as shown from agarose gel (upper panel), and IC50 value (lower panel).
Article Snippet: The antiproliferative activity of purified AME was evaluated towards the liver carcinoma (HepG-2) (ATCC, HB-8065), breast carcinoma (MCF7) (ATCC, HTB-22), human colorectal carcinoma cell line (Caco-2) (ATCC, HTB-37), in addition to the Oral Epithelial Cells (OEC) by the MTT reagent .
Techniques: Purification, Activity Assay, Positive Control, Inhibition, Agarose Gel Electrophoresis